ࡱ> WYV bjbj 7T?  8L5W (!$|J  Q | "+rpUkos~j' 0W %%%W % :  Centrifuge Operation 3/24/08PSESREQUIRED AND RECOMMENDED PERSONAL PROTECTIVE EQUIPMENT: Safety glasses or other depending upon material being centrifugedSEQUENCE OF LABORATORY PROCEDURE POTENTIAL LABORATORY ACCIDENTS OR HAZARDS RECOMMENDED SAFE LABORATORY PROCEDURE Examples:Beware of being too detailed; record only the information needed to describe each job action. Rule of thumb, no more than 10 steps/procedure being evaluatedExamples: Exposures to eyes and skin (splashes, particulates, fumes, lasers), respiratory exposure, ingestion, contact with sharps (broken glass, needles, syringes, sharp metal edges, etc), explosions, uncontrolled energetic reactions, hazardous materials spill, burns, electric shock, slip, trip, or fall, overexertion, ergonomic (awkward postures, excessive force, vibration, repetitive motion),excessive noise.Engineer out New way to do procedure Change physical conditions Change the work procedures Adjust, modify, or replace work station components/tools Decrease task performance frequency Personal Protective Equipment (PPE) Safety glasses, goggles, face shield Gloves Aprons, lab coats, Tyvek Protective foot wear Respiratory protection* Training Protocol specific (by PI) General lab safety or biosafety (EHS or department) Use of Lab Safety Equipment Chemical fume hood Biological safety cabinet Safety shieldOther Improve laboratory housekeeping and hygiene Substitution of less hazardous materials Use alternative methods Use smaller quantities of hazardous materials  Consult instruction manual for specific centrifuge setup, operation guidelines, and maintenance Centrifuge has not been maintained. Chemicals used are incompatible with type of centrifuge Use procedures outlined in centrifuge manual and are specific to the centrifuge to be used. Only use centrifuge that is appropriate for specific chemical hazards of samples. Make sure centrifuge to be used has been maintained properly and is in working order. Identify centrifugal force needed for material based on revolutions per minute (RPM) and radius of centrifuge Centrifugal force or RPM needed is out of the range of the centrifuge Use appropriate centrifuge for centrifugal force needed and weight/volume of samples. Use minimum centrifugal force and time needed to accomplish task. Put material to be centrifuged in appropriate containers Container may break causing the spillage of chemicals. Centrifuge may become unbalanced causing damage to the centrifugeMake sure centrifugation container does not show any signs of stress, is chemically compatible, and is suitable for centrifugal forces / RPMSecure appropriate rotor in centrifugeWrong rotor or rotor is not secured properly resulting in personal injury or damage to the centrifugeConsult owners manual for instructions on securing rotor properly. Type of rotor should be compatible with expected centrifugal force or RPM Place containers in centrifuge cups Unbalancing of centrifuge, personal injury, and damage to centrifuge and other equipmentMake sure sample containers are made to fit in centrifuge cups. Make sure cups are identical and rubber inserts (if applicable) are placed in the bottom of cups. Balance centrifuge cups with containers using a two-pan balance. Place balanced containers in opposite rotor positionsUnbalancing of centrifuge, personal injury, and damage to centrifuge and other equipmentMake sure balanced containers are in opposite positions. Some water may be added to centrifuge cups to fine-tune balance of samples.Close and latch centrifuge lid Personal injury and damage to centrifugeMake sure lid is closed and securely latched before starting centrifugeTurn centrifuge on and select desired speed (rpm)Rpm is too high for sample containers or centrifuge damage to centrifuge or personal injuryCheck to see desired centrifugal force matches speed/RPM selected Once desired time has elapsed, turn centrifuge off. Let centrifuge come to a complete stop or use brake to stop centrifuge Lid is opened before centrifuge has come to a complete stop. Personal injury from rotating rotor and exposure to leaking chemicalsMake sure centrifuge has come to a complete stop before unlatching lidOpen lid and remove samples Exposure to leaking / broken samplesMake sure samples and sample containers are intact and no liquid has spilled before removing samples     ý鶹 LABORATORY HAZARD ANALYSIS FORM -Page  PAGE 1 of  NUMPAGES 2- * Anyone who must wear respiratory protection as a part of their job must participate in the universitys respiratory protection program. Please call Environmental Health and Safety at 885-6524 for more information. 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