ࡱ> @B=>? Objbj ehhG  MMMMM$qqqq%DqOiiiiiDZOOOOOOO$=QS8OMDD8OMMiiMO"MiMiOOHXLi~`Cq J(NcO0O*JTZTPLLTML48O8OPZOT  : ý鶹 Sandpoint Research & Extension Center LABORATORY SAFETY PLAN Introduction - The ý鶹 recognizes and supports the importance of laboratory teaching and research activities conducted at the university. The university also recognizes the many different applications hazardous materials have in an institutional environment. The purpose of this Laboratory Safety Plan is to promote these activities while providing procedures that will ensure the safety of personnel working in laboratories when handling hazardous chemicals, radioactive materials, biological agents, or while operating certain types of equipment that could cause physical harm. Users must perform a hazard assessment prior to operations and determine the nature of all hazards and use appropriate control measures to mitigate the hazards present. This Laboratory Safety Plan is specific to Sandpoint Research & Extension Center (SREC) laboratory. Contact Information: Primary Contact:Danny L. Barney, Ph.D.Phone:Office 208-263-2323 Cell: 208-255-9370 Home: 208-263-2552Department:Plant, Soil, & Entomological Sciences Chemicals - Appendix A contains a general list of chemicals stored and/or used in the SREC laboratory. A chemical inventory is maintained electronically and is stored on the hard drive of Dr. Barneys desktop computer and backed up on an external hard drive. The inventory can be accessed by Dr. Barney or the SREC Administrative Assistant, Brooke Ramsey. The file location is C:\My Documents\RESEARCH\Laboratory Protocols\Inventories\Chemical Inventory.xls. Material Safety Data Sheets- Material safety data sheets (MSDSs) are an essential source of information regarding the chemical composition and hazards presented by a material.MSDS information includes physical data, toxicity and health effects, first aid recommendations, reactivity data, storage and disposal information, recommended protective equipment, and spill or leak response procedures. An MSDS also provides valuable information for medical personnel in the event of an injury or exposure involving a hazardous material.MSDSs must be current and reflect those materials that are being stored and used in the laboratory. The following website provides MSDS information: http://hazard.com/msds/index.php. MSDSs for laboratory materials are stored electronically on Dr. Barneys desktop hard drive and on an external hard drive and can be accessed by Dr. Barney or Brooke Ramsey. The address is C:\My Documents\R&E-CENT\Safety\MSDS. Standard Operating Procedures- Standard Operating Procedures (SOPs) are contained in Appendix C. Fume Hood The portable fume hood presently in the laboratory is not vented and is not to be used for toxic or hazardous materials. Laminar Flow Hood The laminar flow hood is to be used only for micropropagation of plant materials or seed germination trials. No toxic or hazardous materials, other than 70% ethanol or Lysol disinfectant are to be used in the hood. No open flames are allowed. The hood provides no personal protection and is not to be used with pathogenic microorganisms. Personal Protective Equipment - The following safety equipment is required for entry and/or working in this laboratory: For ý鶹 personnel, students, and interns who work in the laboratory. Eye protection - Safety glasses must be worn at all times in the laboratory.Safety goggles must be worn when working with chemicals or engaging in activities that could involve splash hazards, flying debris, particulates, dusts, mists, or aerosols. Gloves Heavy-duty latex or nitrile gloves are to be used loading or unloading the autoclave and when washing glassware. Latex or vinyl surgical gloves are to be worn during micropropagation. Footwear - Closed toe shoes are required at all times in the laboratory. Clogs with holes in the shoe uppers are not allowed. When micropropagation is in progress, street shoes are to be removed outside of the laboratory and replaced with clean shoes kept only for laboratory and office use. Clothing A lab coat is required when working with chemicals and biological agents. Lab aprons are available when working with liquids. The laboratory doubles as a clean room. Clean clothes and clean street shoes are required to enter the laboratory. Employees should complete laboratory work before engaging in outdoor or greenhouse activities. Footwear and clothing used in field work, in the greenhouse, or for landscape maintenance are not allowed in the laboratory unless specifically directed by Dr. Barney while plant materials are being transferred into or out of the laboratory. For visitors to the laboratory. Eye protection - Safety glasses must be worn at all times in the laboratory.Safety goggles must be worn when activities involve splash hazards, flying debris, or the generation of particulates, dusts, mists, or aerosols. Footwear - Closed toe shoes are required at all times in the laboratory. Clogs with holes in the uppers are not allowed. ý鶹ors with otherwise unacceptable footwear may enter the lab wearing disposable booties over their street shoes. Clothing - ý鶹ors entering the laboratory must be wearing clean clothes and clean street shoes that have not been worn in a garden, greenhouse, field, forest, or landscape since laundering. First Aid A first aid kit is located in the kitchen area immediately outside the laboratory door. The first aid kit should be checked monthly. The first aid kit should contain, at a minimum, Items listed in the ý鶹 standardized first aid kit guidelines. The list can be found at: www.uidaho.edu/safety/FacilityInspections/FacilityInspectionsHome.htm. A copy of the Blood-Borne Pathogen Safety Guidelines is to remain posted near the first aid kit. Safety Inspections The SREC laboratory will undergo a safety inspection once monthly. The inspection will include a test of the emergency eyewash and emergency shower. A paper copy of the signed inspection form will be filed in the laboratory and an electronic copy maintained on Dr. Barneys desktop computer. Applicability - All personnel who use the ý鶹 laboratory facilities, or who participate in a ý鶹 sponsored activity that involves hazardous materials in laboratories are expected to comply with the provisions of this manual. Personnel including, but not limited to, faculty, staff, teaching and research assistants, and students are responsible for conducting activities in laboratories and handling hazardous materials in a safe manner as specified in this manual. Procedures are to be established in the laboratory safety plan that will provide appropriate protection to non-university personnel, including visitors, tour groups, maintenance and repair personnel, who would have reason to enter the laboratory. All university departments are responsible for ensuring employees are provided with, and understand, a laboratory safety plan applicable for the specific work area. Appendix A Chemicals typically stored and/or used in the SREC laboratory are listed below. A chemical inventory is maintained electronically and is stored on the hard drive of Dr. Barneys desktop computer and backed up on an external hard drive. The inventory can be accessed by Dr. Barney or the SREC Administrative Assistant, Brooke Ramsey. The file location is C:\My Documents\RESEARCH\Laboratory Protocols\Inventories\Chemical Inventory.xls. Product NameProduct DescriptionLocation1, 10 phenanthroline ferrous sulfateChemical Cabinet 12,4 DC8H6Cl2O3Chemical Cabinet 16 aza uracilChemical Cabinet 18-quinolinol hemisulfate saltC9 H7 NO * H2O4SChemical Cabinet 1acetic acid, glacialCH3COOHChemical Cabinet 1acetoneFlammable Cabinet 1acid plant food Chemical Cabinet 2adenine sulfate(C3H5N5)2 *H2SO4 * 2H2OChemical Cabinet 1agarChemical Cabinet 1aluminum ammonium sulfateAl2(SO4)3 * NH4)2SO4-24H2OChemical Cabinet 1ammonium molybdate - 4-hydrate(NH4)6Mo7O24* 4H2OChemical Cabinet 1ammonium nitrateNH4NO3Chemical Cabinet 1calcium sulfate - anhydrousCa SO4Chemical Cabinet 1aniline blueChemical Cabinet 2antimony potassium tartrateK(SbO)C4H4O6 * 1/2H2OChemical Cabinet 1azomethine H boron reagentChemical Cabinet 1barium sulfateBA SO4Chemical Cabinet 1basic fuchsinChemical Cabinet 2benomylChemical Cabinet 2boric acidH3 BO3Chemical Cabinet 1buffer 7+(-) 0.02Chemical Cabinet 2buffer 9+ (-) 0.02Chemical Cabinet 2buffer Solution pH 4.00 (red)Chemical Cabinet 2buffer Solution pH 7.00 (yellow)Chemical Cabinet 2buffer Solution pH10 (blue)Chemical Cabinet 2buffer Tablets (4 pH)Chemical Cabinet 2buffer Tablets (7pH)Chemical Cabinet 2caffeic acidChemical Cabinet 1calcium carbonateCa CO3Chemical Cabinet 1calcium chloride - dihydrateCa Cl2 * 2H2OChemical Cabinet 1calcium nitrateCa (NO3)2 * 4H2OChemical Cabinet 1CalgonChemical Cabinet 1casein hydrolysateChemical Cabinet 1Chamber Cleaner HCLChemical Cabinet 3chlorogenic acidChemical Cabinet 1chromium trioxideCrO3Chemical Cabinet 1citric acidC6H8O7Chemical Cabinet 1Clorox Chemical Cabinet 3cobalt chloride - hexahydrateCo Cl2 * 6H2OChemical Cabinet 1colchicineChemical Cabinet 1copper carbonate (basic)Chemical Cabinet 1crystal violetChemical Cabinet 2cupric sulfateChemical Cabinet 1dimethyl sulfoxide(CH3)2 SOChemical Cabinet 1ethanolC2 H5 OHFlammable Cabinet 1ethylenediaminetetraacetic acid iron (III) sodium saltC10H12FeN2NaO8Chemical Cabinet 1ethylenediaminetetraacetic acid - disodium saltC10H14N2O6Na2 * 2H2OChemical Cabinet 1fast green Chemical Cabinet 2ferric ammonium sulfate Fe Nh4 (SO4)2 * 12H2OChemical Cabinet 1ferrous sulfate - heptahydrateFeSO4 * 7H2OChemical Cabinet 1ferulic acidChemical Cabinet 1formaldehydeH CHOChemical Cabinet 3gelatinChemical Cabinet 2gibberellic acid - potassium saltC19 H21 K O6Chemical Cabinet 2glycerolHO CH2CHO HCH2OHChemical Cabinet 2glycine HClCa Hg N2 O3 * HCLChemical Cabinet 2hematoxylin - trihydrateChemical Cabinet 2Hemo DeFlammable Cabinet 1hydrochloric acidHCLChemical Cabinet 3hydrogen peroxideH2O2Chemical Cabinet 3indole 3-butyric Acid (0.1%)Chemical Cabinet 2lacmoid (resorcin blue)Chemical Cabinet 2lactic acid (85%)Chemical Cabinet 3L-ascorbic acid C6H8O6Chemical Cabinet 2magnesium chloride - hexahydrateMg Cl2 * 6H2OChemical Cabinet 2magnesium perchlorateChemical Cabinet 2magnesium sulfate - heptahydrateMg SO4 * 7H2OChemical Cabinet 2manganese sulfate - anhydrousMn SO4Chemical Cabinet 2manganese sulfate - monohydrateMnSO4 H2OChemical Cabinet 2manganese chloride - 4-hydrateMn Cl2 * 4H2OChemical Cabinet 2mercuric chlorideHg ClChemical Cabinet 2methyl cellulose powderChemical Cabinet 2methylene blueChemical Cabinet 2myo InositolC6 H12 O6Chemical Cabinet 2nicotinic acidChemical Cabinet 2nitric acidH NO3Chemical Cabinet 3oxalic acid(COOH)2 * 2H2OChemical Cabinet 2ParaplastChemical Cabinet 3pH paper (6 to 8)Chemical Cabinet 2phenolC6 H5 OHChemical Cabinet 2PhytagelChemical Cabinet 2picric acid, Saturated (1.2%)Chemical Cabinet 3potassium chloride KCLChemical Cabinet 2potassium dichromateChemical Cabinet 2potassium hydroxideKOHChemical Cabinet 2potassium iodideKIChemical Cabinet 2potassium nitrateK NO3Chemical Cabinet 2potassium permanganateK MnO4Chemical Cabinet 2potassium phosphate - monobasicK H2 PO4Chemical Cabinet 2potassium sulfate K2 SO4Chemical Cabinet 2Randolphs microscopy fixativeSolution AChemical Cabinet 2Randolphs microscopy fixative Solution BChemical Cabinet 2safranin O Chemical Cabinet 2soda limeChemical Cabinet 2sodium acetate - anhydrousNa C2 H3 O2Chemical Cabinet 2sodium acetate - trihydrateNa C2 H3 O2 * 3H2OChemical Cabinet 2sodium chlorideNa ClChemical Cabinet 2sodium hydroxideNa OHChemical Cabinet 2sodium molybdate - dihydrateNa Mo2 * 2H2OChemical Cabinet 2sodium nitrateNa NO3Chemical Cabinet 2sodium phosphate - monobasic Na H2 PO4 * H2OChemical Cabinet 2sodium phosphate - dibasic Na HPO4 * 12H2OChemical Cabinet 2orange GChemical Cabinet 2Standard Wetting Agent (Tween20)Chemical Cabinet 2sucroseC12H22O11Chemical Cabinet 2sulfuric acidChemical Cabinet 3tannic acidChemical Cabinet 2thiamine HClChemical Cabinet 2thidiazuronFreezerthymol, crystalChemical Cabinet 2tolueneC6H5CH3Flammable Cabinet 1Triton X-100 (wetting agent)Chemical Cabinet 2Woody Plant Medium Refrigeratorzinc chlorideZn Cl2Chemical Cabinet 2zinc sulfate - heptahydrateZn SO4 * 7H2OChemical Cabinet 2 Appendix B Material Safety Data Sheets Material Safety Data Sheets for the SREC are maintained electronically on Dr Barneys desktop hard drive with external hard drive backup. The files can be accessed by Dr. Barney or Brooke Ramsey at C:\My Documents\R&E-CENT\Safety\MSDS. MSDSs can also be accessed online at http://hazard.com/msds/index.php. Appendix C Standard Operating Procedures Laboratory Procedure: Laminar hood operationDATE: April 10, 2008Department: Sandpoint Research & Extension CenterLABORATORYAnalysis by: Danny L. BarneyREQUIRED AND RECOMMENDED PERSONAL PROTECTIVE EQUIPMENT:APPROVED BY: Danny L. BarneySEQUENCE OF LABORATORY PROCEDUREPOTENTIAL LABORATORY ACCIDENTS OR HAZARDSREQUIRED SAFE LABORATORY PROCEDUREUsing a hand spray bottle, spray the inside plastic walls, countertop, and bacticinerator or glass bead sterilizer with an aqueous solution of Lysol. Do not spray the perforated back wall of the hood with Lysol. Disinfect the back wall of the hood with a spray of 70% pure ethanol. Do not use denatured ethanol. Avoid spraying the ethanol near the bacticinerator or glass bead sterilizer. Turn on the hood blower and bacticinerator or glass bead sterilizer.Flash fire. Inhalation of alcohol fumes.Wear splash goggles. Wear lab coat. Wear latex or vinyl surgical gloves.Place a sterilized instrument rack, instruments, and paper towel into hood. Spray the outsides of the sterilization bags with a 70% solution of pure ethanol. Flash fire. Inhalation of alcohol fumes.Wear splash goggles. Wear lab coat. Wear latex or vinyl surgical gloves.During micropropagation or seed germination work, frequently disinfect hands by spraying with 70% ethanol. Between cultures, spray the countertop, instruments, and rack with 70% ethanol. Between containers, disinfect instruments by placing the tips into the bacticinerator or glass bead sterilizer for 20 seconds and then replacing the instruments in the rack to cool. Do not place instruments in the bacticinerator or glass bead sterilizer if they have any alcohol on them or you have alcohol on your hand. Do not spray alcohol near bacticinerator.Flash fire. Inhalation of alcohol fumes.Wear splash goggles. Wear lab coat. Wear latex or vinyl surgical gloves.Disinfect containers being brought into the hood with a wetting spray of 70% ethanol.Flash fire. Inhalation of alcohol fumes.Wear splash goggles. Wear lab coat. Wear latex or vinyl surgical gloves. Laboratory Procedure: Steam sterilizer operationDATE: April 9, 2008Department: Sandpoint Research & Extension CenterLABORATORYAnalysis by: Danny L. BarneyREQUIRED AND RECOMMENDED PERSONAL PROTECTIVE EQUIPMENT:APPROVED BY: Danny L. BarneySEQUENCE OF LABORATORY PROCEDUREPOTENTIAL LABORATORY ACCIDENTS OR HAZARDSREQUIRED SAFE LABORATORY PROCEDUREIf the water tank is one-half full or less, set the sterilizer to DRY position, wait until the pressure gauge reads 0, and open the screw stopper in the funnel at the back of the sterilizer. Add cold tap water until the level reads at the top of the sight glass.Contact burns from hot surfaces on sterilizer. Burns from escaping steam. Explosive release of steam pressure.Wear splash goggles.Ensure stopper on funnel is closed tightly and sterilizer is set to OFF. Empty and replace the plastic water collection jar beneath sterilizer. Turn switch at lower left rear of sterilizer to ON. Allow the jacket pressure to build to 17 to 20 pounds per square inch of pressure.Contact burns from hot surfaces on sterilizer. Burns from escaping steam. Explosive release of steam pressure.Wear splash goggles.Ensure that the chamber pressure gauge reads 0. Stand in front of the sterilizer and to the right, away from the door. Engage the safety latch and slowly rotate the latch wheel counterclockwise to a fully open position. Then disengage the safety latch and open the door. Load the chamber so as to leave room around and between items. Reengage the safety latch and turn the latch wheel clockwise until it seats firmly.Contact burns from hot surfaces on sterilizer. Burns from escaping steam. Explosive release of steam pressure. Door can swing open violently.Wear splash goggles and heavy-duty latex or nitrile gloves.Stand to the right side of the sterilizer away from the door. Set the sterilizer to Sterilize. Allow the chamber pressure to build to 17 to 20 pounds per square inch of pressure. Allow contents to remain at that pressure for 35 minutes.Contact burns from hot surfaces on sterilizer. Burns from escaping steam. Explosive release of steam pressure. Door can swing open violently.Wear splash goggles and heavy-duty latex or nitrile gloves.If the Chamber contains liquids, set the sterilizer to SLOW EXHAUST and allow the chamber pressure to drop to 0 before opening the door as described above. If there are no liquids or sealed containers inside the chamber, the sterilizer can be set to FAST EXHAUST and allowed to depressurize before opening, as described above. If you are finished with the sterilizer, set it to DRY to relieve the jacket pressure, leaving the door closed with the safety latch engaged but the door loose.Contact burns from hot surfaces on sterilizer. Burns from escaping steam. Explosive release of steam pressure. 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