Instruments
Bruker AVANCE III HD 500 MHz
This is a new (2016) instrument equipped with a broadband SMART probe and with a PRODIGY broadband cold probe. With the PRODIGY probe the NMR coil assembly and the preamplifier are cooled by evaporating liquid nitrogen. This achieves extremely efficient operation of the NMR coil and significantly reduces thermal noise resulting in the enhance of the overall signal-to-noise ratio compared to standard room temperature probes. The experiment time is thereby dramatically reduces (about 4 times). Both probes are equipped with a z-gradient assembly. Here are some examples of a time needed to run various NMR experiments with a typical organic sample with MW over a range of 200 – 800:
- 1H NMR (3mg) - < 5 min
- 13C{1H} NMR (10 mg) – 10-15 min
- 15N NMR (40 – 60 mg) – 1-3 hours
- 1H-1H COSY (5mg) – 5 – 10 min (depending of the type of the experiment)
- 1H -13C HSQC or HMBC (5 – 15 mg) – 10-20 min
- 1H-15N HSQC or HMBC (15 – 30 mg) – 15-30 min
- 1H-1H ROESY or NOESY (10 – 20 mg) – 4-6 hours
- 13C-13C INADAQUATE (50+ mg) – 10-14 hours
Many other experiments are also available (selective excitation and decoupling, solvent suppression, TOCSY, HSQC-TOCSY, DOSY, etc). This instrument is operated solely by the NMR Lab manager.
Bruker AVANCE 500 MHz
This is a 20 years old spectrometer equipped with a broadband BBO probe (no gradient) and a solid-state broadband MAS probe. The transition from high resolution regime to solid-state takes about 30 min. This instrument is operated by qualified UI users (high resolution NMR) or by the Lab manager (solid-state NMR).
Bruker Avance 300 MHz
The spectrometer is equipped with a QNP probe (4-nuclei: 1H, 13C, 31P and 19F). The instrument is operated solely by qualified UI users on the first-come basis. Overnight experiments start not earlier than 9 pm and should be pre-reserved. The instrument is used for routine NMR analysis (1D spectra, DEPT, fast-COSY).
Bruker EPR EMX
This is a standard EPR spectrometer with a 3 cm cavity. An additional insert is available to perform experiments with water solutions. The real-time monitoring of radical reaction is available. The liquid He thermostat assembly allows low-temperature (down to 4 K) experiments.